Abstract
BackgroundFollowing peroral Toxoplasma (T.) gondii infection, susceptible mice develop acute ileitis due to a microbiota-dependent Th1 type immunopathology. Toll-like-receptor (TLR)-9 is known to recognize bacterial DNA and mediates intestinal inflammation, but its impact on intestinal microbiota composition and extra-intestinal sequelae following T. gondii infection has not yet been elucidated.Methods and resultsSeven days following peroral infection (p.i.) with 100 cysts of T. gondii ME49 strain, TLR-9-/- and wildtype (WT) mice suffered from comparable ileitis, whereas ileal parasitic loads as well as IFN-γ and nitric oxide levels were higher in TLR-9-/- compared to WT mice. Locally, TLR-9-/- mice exhibited increased ileal CD3+, but not FOXP3+ cell numbers at day 7 p.i.; in mesenteric lymph nodes IFN-γ-producing CD4+ cell numbers and TNF-α and IFN-γ concentrations were also increased in TLR-9-/- compared to WT mice. T. gondii DNA levels, however, did not differ in mice of either genotype. Differences in intestinal microbiota were rather subtle except for bifidobacteria that were virtually absent in both, naïve and T. gondii infected TLR-9-/-, but not WT mice. Extra-intestinally, TLR-9-/- mice displayed less distinct systemic immune responses as indicated by lower serum IL-6, and splenic TNF-α and IFN-γ levels as compared to WT mice despite higher translocation rates of intestinal bacteria to extra-intestinal compartments such as liver, spleen, kidney, and cardiac blood. Most importantly, brains were also affected in this inflammatory scenario as early as day 7 p.i. Remarkably, TLR-9-/- mice exhibited more pronounced inflammatory infiltrates with higher numbers of F4/80+ macrophages and microglia in the cortex and meninges as compared to WT mice, whereas T. gondii DNA levels did not differ.ConclusionWe here show that TLR-9 is not required for the development of T. gondii induced ileitis but mediates distinct inflammatory changes in intestinal and extra-intestinal compartments including the brain.
Highlights
Seven days following peroral infection with 100 cysts of Toxoplasma (T.) gondii ME49 strain, susceptible mice develop massive necrosis in the terminal ileum and succumb to the infection [1]
We here show that TLR-9 is not required for the development of T. gondii induced ileitis but mediates distinct inflammatory changes in intestinal and extra-intestinal compartments including the brain
Acute ileal immunopathology in TLR-9−/− mice following peroral T. gondii infection In order to induce acute ileitis TLR-9−/− and wildtype (WT) mice were subjected to a peroral infection with 100 cysts of T. gondii ME49 strain
Summary
Seven days following peroral infection with 100 cysts of Toxoplasma (T.) gondii ME49 strain, susceptible mice develop massive necrosis in the terminal ileum and succumb to the infection [1]. This fatal scenario is due to a classical Th1-type immunopathology orchestrated by intestinal epithelial cells, granulocytes, macrophages, monocytes, dendritic cells, and lymphocytes [2]. Parasitic DNA and RNA have been shown to activate innate immune responses via TLR-7 and TLR-9, but mice lacking TLR-9 alone were not susceptible to T. gondii infection [19]. Following peroral Toxoplasma (T.) gondii infection, susceptible mice develop acute ileitis due to a microbiota-dependent Th1 type immunopathology. Toll-like-receptor (TLR)-9 is known to recognize bacterial DNA and mediates intestinal inflammation, but its impact on intestinal microbiota composition and extra-intestinal sequelae following T. gondii infection has not yet been elucidated
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