Abstract
AbstractIn Lepidium virginicum L., exposure of pollen to 0.6 ppm sulfur dioxide (SO2) for 4 h reduced pollen germination in vitro 94% from the control, whereas exposure to 0.6 ppm SO2 for 2, 4, and 8 h during flowering reduced pollen germination in vivo 50% from the control, but did not affect seed set. An interaction between SO2 and water may have caused the inhibition of pollen germination in a liquid culture medium, as well as on the moist surface of an intact stigma. However, the results suggest that the use of pollen germination and pollen tube elongation in vitro to assess the direct effects of SO2 on plant sexual reproduction in vivo is not valid.
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