The Impact of Salinity, Putrescin, and Melatonin Interactions on Green Bean Vegetative Indicators

Abstract

This study aims to assess the effect of salt stress by using concentrations of sodium chloride (0, 40, 20, 60) mmol. L-1. Putrescine (0.2 and 0.4) mmol. L-1 and Melatonin (0.2, 0.4) mmol. L-1 and their overlap in some physiological and phenotypic traits of a green bean plant. The experiment was carried out using a complete randomized design (CRD) as a two-factor experiment (putrescine and melatonin x sodium chloride salt concentrations). The results were statistically analyzed with 10 replications that included 20 root tips for each treatment, and the averages were compared using the least significant difference at the 0.05 probability. The results showed that the effect of salt stress led to a significant decrease in most of the studied traits, a concentration of 60 mmol. L-1 led to a decrease in the percentage of plant survival by average branch length, number of shoots, dry weight percentage, and chlorophyll content. Also, adding melatonin significantly increased most of the studied traits using concentrations of 0.2 and 0.4 mmol. L-1 compared to adding putrescin (0.2), as the average branch length increased by increase, the number of shoots, the survival rate of plants, the dry weight, and the percentage of chlorophyll. The experiment’s results also showed a significant effect of the interaction between salt stress and melatonin on most of the vegetative growth characteristics of green beans. The effect of putrescine led to a decrease in vegetative indicators due to its high concentration in the medium used in the experiment.