Abstract
ObjectiveTo investigate the effect of triglyceride (TG) integrates with plasma major components of apolipoproteins in HDL subclasses distribution and further elicited the TG-apolipoproteins (apos) interaction in the processes of high density lipoprotein (HDL) mature metabolic and atherosclerosis related diseases.MethodsContents of plasma HDL subclasses were quantities by two-dimensional gel electrophoresis associated with immunodetection in 500 Chinese subjects.ResultsContents of preβ1-HDL, HDL3a, and apoB-100 level along with apoB-100/A-I ratio were significantly increased, whereas there was a significant reduction in the contents of HDL2, apoA-I level as well as apoC-III/C-II ratio with increased TG concentration. Moreover, preβ1-HDL contents is elevated about 9 mg/L and HDL2b contents can be reduced 21 mg/L for 0.5 mmol/L increment in TG concentration. Moreover, with increase of apoA-I levels, HDL2b contents were marginally elevated in any TG concentration group. Furthermore, despite of in the apoB-100/A-I < 0.9 group, the contents of preβ1-HDL increased, and those of HDL2b decreased significantly for subjects in both high and very high TG levels compared to that in normal TG levels. Similarly, in the apoB-100/A-I ≥ 0.9 group, the distribution of HDL subclasses also showed abnormality for subjects with normal TG levels.ConclusionsThe particle size of HDL subclasses tend to small with TG levels increased which indicated that HDL maturation might be impeded and efficiency of reverse cholesterol transport(RCT) might be weakened. These data suggest that TG levels were not only significantly associated with but liner with the contents of preβ1-HDL and HDL2b. They also raise the possibility that the TG levels effect on HDL maturation metabolism are subjected to plasma apolipoproteins and apolipoproteins ratios.
Highlights
Several studies were published in the 1970s reporting an inverse association between plasma high density lipoprotein cholesterol (HDL-C) level and coronary heart disease (CHD) [1], confirmed since by numerous additional studies throughout the world
Concentrations of plasma lipids, apolipoproteins, and ratios of lipids and apolipoproteins among subjects classified by levels of TG Table 1 showed that in the population, the concentration of TG was 2.1 ± 0.3 mmol/L which exceeds normal TG levels recommended in the Adult Treatment Panel III (ATP-III) of National Cholesterol Education Program (NCEP) guidelines that indicated a substantial proportion of subjects with hypertriglyceridemia (HTG)
The body mass index(BMI) was increased with the elevation of the TG levels and the subjects in borderline-high, high and very high TG groups were characterized by an adverse lipid profile which comprised of increased concentrations of TG, total cholesterol (TC), low density lipoprotein cholesterol(LDL-C) and levels of apoB-100, apoC-II, and apoC-III along with the ratios of TG/HDL-C, TC/HDL-C,LDL-C/HDL-C, and apoB-100/A-I; lower levels of HDL-C, apoA-I and apoC-III/C-II ratio compared with normal TG subjects
Summary
Several studies were published in the 1970s reporting an inverse association between plasma high density lipoprotein cholesterol (HDL-C) level and coronary heart disease (CHD) [1], confirmed since by numerous additional studies throughout the world. Using two-dimensional gel electrophoresis coupled with immunoblotting, HDL can be divided into large, cholesterol-rich (HDL2a and HDL2b), small-sized (HDL3c, HDL3b, HDL3a, and preb1HDL) and preb2-HDL [11,12]. Epidemiological studies have shown that individual HDL subclasses are not atheroprotective [13], a decrease content of the large-sized HDL2b particles and an increase content of the small-sized preb1-HDL particles were highly and significantly associated with the risk of CHD [14,15]
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