Abstract
In this research, the aim was to investigate the influence of pH value in the extraction medium on the phytochemical composition and antioxidant activity of Artemisia herba-alba species used in traditional medicine. For this reason, two analytical methods were used (UV-visible spectroscopy, HPLC). Antioxidant properties of the extracts at different pH were estimated by two tests: total antioxidant capacity (TAC) and DPPH free radical scavenging capacity. The results of the evaluation of phenolic compounds showed that the aqueous extracts in a weak acid medium had a high percentage of phenol content, and the highest value for total phenol content was 90.759±1.267mg GAE/g. Whereas, the HPLC analysis indicated the presence of most of the standard phenolic compounds in the majority of the extracts. For antioxidant activity, the best antioxidants reported in extract at pH=6, with a semi-maximal inhibitory concentration (0.151±0,032mg/ml) and a total antioxidant capacity (TAC) of 66.840±0.241mg EAA/g, which indicates that it is effective antioxidant for therapeutic applications.
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