Abstract
Background: The impact of estrogen deficiency and occlusion in the matrix metalloproteinases (MMPs) expression in dental tissues has not yet been elucidated. Objective: To evaluate the influence of estrogen deficiency and occlusal hypofunction and hyperfunction on the gene expression of MMP1, MMP8 and MMP13 in the odontogenic region of teeth in continuous growth, in the murine model. Material and methods: Rats (Wistar Hannover lineage) were divided into two groups according to the intervention received: Hypoestrogenism Group - ovariectomy surgery and Control Group - fictitious surgery. Occlusal hypofunction and hyperfunction conditions were also established in all animals (each animal presented both conditions). After euthanasia, the hemimandibles were removed to evaluate the gene expression through real time PCR. T-test was used to compare the mean differences between groups (P<0.05). Results: There was no statistically significant difference of the relative gene expression of MMP1, MMP8 and MMP13 between the hypoestrogenism and control groups (P>0.05). A statistically significant difference of the relative MMP13 expression between the occlusal hypofunction and hyperfunction tooth was observed (P=0.03). In the hypoestrogenism group, MMP13 was overexpressed in hypofunction tooth (P=0.045). Conclusion: Occlusal function affects MMP13 expression in the odontogenic region, in murine model.
Highlights
Estrogen is a steroidal hormone with lifelong production in the human body
The influence of estrogen and estrogen receptors on the expression of matrix metalloproteinases (MMPs) has already been described in chondrocytes (Lee et al, 2003), osteoblasts (Schiltz et al, 2008), fibroblasts of epithelial tissue (Philips & Devaney, 2003), stromal endometrial cells (Kokorine et al, 1996), and endothelial cells involved in breast carcinogenesis (Nilsson et al, 2007), among others
The analysis was performed in the survival animals: Hypoestrogenism Group (n=8) and Control Group (n=9)
Summary
Estrogen is a steroidal hormone with lifelong production in the human body. In addition to the well-established relationship in male and female reproduction and in several other systems of the human body, estrogen has been associated with several conditions, such as obesity, metabolic disorder, cancers, osteoporosis, lupus erythematosus, endometriosis, and uterine fibroids (Patel et al, 2018). The increase in the MMPs levels has been described before under estrogen deficiency condition, known as hypoestrogenism (Lee et al, 2003). The impact of estrogen deficiency and occlusion in the matrix metalloproteinases (MMPs) expression in dental tissues has not yet been elucidated. Objective: To evaluate the influence of estrogen deficiency and occlusal hypofunction and hyperfunction on the gene expression of MMP1, MMP8 and MMP13 in the odontogenic region of teeth in continuous growth, in the murine model. Results: There was no statistically significant difference of the relative gene expression of MMP1, MMP8 and MMP13 between the hypoestrogenism and control groups (P>0.05). A statistically significant difference of the relative MMP13 expression between the occlusal hypofunction and hyperfunction tooth was observed (P=0.03). Conclusion: Occlusal function affects MMP13 expression in the odontogenic region, in murine model.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
