Abstract

Methane production represents an important sink for hydrogen within the rumen Beever (1993) suggested that the partitioning of fermentable dry matter (DM) between microbial synthesis and fermentation products would alter the pattern of hydrogen production and hence methanogenesis. This hypothesis was investigated in vitro using a range of diets varying in carbohydrate source (Moss et al., 2000). Methane production (moles) increased as the proportion of DM fermented to short chain fatty acids (SCFA) increased and this was related to decreasing water soluble carbohydrate (WSC) to cell wall (NDF) ratio of the diet. The objectives of the current study was to design diets with a range of WSC:NDF ratios and to measure the impact on hexose partitioning and methane production in sheep in vivo.

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