Abstract
Inoculation of Streptomyces to improve oilseed rape (Brassica napus L.) yields and minimise the use of chemical fertilisers is a promising sustainable strategy. In this study, we isolated 72 actinobacterial strains from rhizosphere of oilseed rape and maize and from bulk soil for screening and characterising their antimicrobial activity. Nine promising strains, identified as Streptomyces sp. by morphology, physiological characteristics, and 16S rRNA gene sequencing, were selected for their plant growth-promoting traits and in planta experiments. The actinobacterial strains were positive for IAA production, siderophore production, and HCN production. In planta experiments were conducted by soaking the oilseed rape seeds in the actinobacterial suspension, followed by plant growth under controlled conditions in a cultivate chamber (22–28 °C, 8 h dark/16 h light, constant humidity 80%). We recorded root and shoot length (cm) and seedling fresh weight (g). For most of the abovementioned parameters, a significant enhancement was observed with strain KmiRC20A118 treatment. The length of the root increased by 53.14%, the shoot length increased by 65.6%, and the weight of the fresh plant by 60% compared to the control. The integrated application of PGPS (Plant Growth Promoting Streptomyces) from the rhizosphere of oilseed rape is a promising strategy to improve the growth of oilseed rape.
Highlights
Beneficial plant-associated microorganisms are a valuable resource for the development of biological control agents and plant growth-promoting rhizobacteria (PGPR) [6]
The tested bacteria and fungi were among the important plant pathogens of wheat, potatoes, corn, beans, oilseed rape, and many others [32,33]
This study confirmed our hypothesis that streptomycetes strains from the rhizosphere of oilseed rape showed promoting streptomycetes (PGPS) traits and significantly promoted oilseed rape growth under controlled conditions
Summary
Oilseed rape (Brassica napus L.) is one of the valuable oilseed crops. It has been attracting attention in recent years as an important oil-producing plant [1] and as an alternative renewable energy source [2]. Oilseed rape plants lack resistance to most soil-borne pathogens and are reliant on microbial-based defence mechanisms for their survival [5]. Beneficial plant-associated microorganisms are a valuable resource for the development of biological control agents and plant growth-promoting rhizobacteria (PGPR) [6]. Actinobacteria are associated with the biological control of insect pests and pathogens, stress tolerance and growth promotion in plants [8]
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