Abstract
Gold nanoparticles (AuNPs) have gained significance due to their size and shape. The current study aimed to prepare AuNPs by using green - plasma jet and calculate the cellular toxicity of those manufactured nanomaterials. Aqueous gold tetrachloride salts (HAuCl<sub>4</sub>&#183;4H<sub>2</sub>O) and essential oil of lemon peels in different ratios (0:10, 1:9, 2:8, and 3:7) were used to produce AuNPs. The synthesized green-prepared gold was characterized by ultraviolet-visible (UV-vis), X-ray diffraction (XRD), and field emission scanning electron microscopy (FESEM). UV-vis spectrum showed the highest absorption at 540 nm, and the patterns of XRD at the ratio (2:8) showed four peaks occurred at peaks at 2&theta; &#61; 38.1, 44.3, 64.5, and 77.98. FESEM study illustrated that the AuNPs particle sizes range between (38-76 nm). The cell toxicity results have shown the effectiveness of the green synthesized AuNPs on the breast cancer (AMJ13) cell line. The breast cancer (AMJ13) cell line is found to be more inhibited when the concentration of AuNPs reaches 100&#37;. According to the findings, creating nanoparticle materials for the therapy of breast cancer cells using green-cold plasma is a potential technique.
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