Abstract
The aim of this research is to evaluate the effect of glucose and sodium chloride on biofilm formation by bacteria causing wound infection. For this purpose, 1% and 2% concentration of each of glucose and sodium chloride were used to test the biofilm formation potential of Staphylococcus aureus and Pseudomonas aeruginosa, which were the most common abundant bacteria that cause infection by biofilm. Each of the concentrations was kept in contact with the pathogenic bacteria for 24 hours. After the period of incubation, the concentration of 1% of glucose enhanced moderate biofilm formation capacity for (66% and 80%) on both bacteria respectively. The concentration of 2% glucose, on the other hand, led to a weak biofilm for 33% and 20% on both bacteria isolates respectively. In respect to the effect of sodium chloride, no isolate was able to form neither moderate nor strong biofilms. Nonetheless, all isolates succeeded in forming weak biofilms at 2% sodium chloride, while treatment with a concentration of 1% sodium chloride led to inhibited biofilm formation for 43% of isolates. Besides, Pseudomonas aeruginosa isolates were able to form moderate biofilms in the presence of 1% concentration of glucose, and weak producers in the presence of 2% glucose concentration. The isolates succeeded in forming strong biofilms at both 1% and 2% sodium chloride.
Highlights
Bacterial biofilms are regarded as the major cause of chronic wound infections
Biofilm construction is a secure adjusting type of thriving that allows bacteria to survive in inhospitable conditions like those of a human body and to propagate and spread their requirements, which are facilitated by their molecular crosstalk known as Quorum Sensing (QS) [3]
The matrix of bacterial biofilm is made of different polymers such as polysaccharide, proteins, and extracellular DNA .The formation of biofilms is a procedure that goes through multiple stages, the first of which is a provisional attachment to the surface
Summary
Bacterial biofilms are regarded as the major cause of chronic wound infections. Their high resistance against antibiotic treatments makes their annihilation more challenging. Bacteria agglutinate and form extracellular polymeric substances during the step of accumulation [5] This process of biofilm construction relies on various factors such as the properties and the nature of the carbon source, its concentration, pH, ionic strength, and temperature [6]. We aim in this study to establish a consensus method to attain the maximum possible in vitro biofilm construction using clinical isolates from wound infection bacteria consuming the supplementations with the suitable concentration of glucose and sodium chloride
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