Abstract
Objective To observe the effect of electroacupuncture (EA) pretreatment of different durations on the expressions of AQP4 and CLN5 in the blood-brain barrier after cerebral ischemia and reperfusion Methods Sixty-four male Sprague-Dawley (SD) rats were randomly divided into a model group, a sham operation group, a 7-day EA-pretreatment group (EAP-7 group) and a 15-day EA-pretreatment group (EAP-15 group), each of 16. After EA-pretreatment on the baihui and shuigou acupoints for 7 days or 15 days, a model of unilateral middle cerebral artery embolism (MCAO) model was induced in the rats of the model, EAP-7 and EAP-15 group using a modified Longa method. In the sham operation group the carotid artery was separated without middle cerebral artery embolism. The reperfusion was begun 90 min after the MCAO modeling. Immunohistochemical methods and fluorescence quantitative PCR were applied to detect the expression of AQP4, CLN5 and their mRNAs expression in the blood brain barrier (BBB) 24h after the operation. Results Compared with the sham operation group, the expression of AQP4 positive cells and AQP4 mRNA in the BBB in the other three groups had increased significantly, while the expression of CLN5 positive cells and CLN5 mRNA was significantly less. Compared with the model group, the expression of AQP4 positive cells and AQP4 mRNA in the EAP-7 and EAP-15 groups was significantly reduced, while the expression of CLN5 positive cells and CLN5 mRNA was significantly increased. Moreover, the expression of AQP4 positive cells and AQP4 mRNA in the EAP-15 group were significantly higher than in the EAP-7 group, while the expression of CLN5 positive cells and CLN5 mRNA were significantly lower. Conclusions EA-pretreatment on the baihui and shuigou acupoints can restrain the expression of AQP4-positive cells and AQP4 mRNA and promote that of CLN5-positive cells and CLN5 mRNA in the BBB after cerebral ischemia and reperfusion, at least in rats. The BBB protection effect is better when the EA-pretreatment lasts longer. The mechanisms of cerebral ischemia tolerance may be related to the regulation of AQP4, CLN5 and their mRNAs in the blood-brain barrier after cerebral injury. Key words: Acupuncture; Cerebral ischemia; Reperfusion; Blood-brain barrier; AQP4; CLN5
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Chinese Journal of Physical Medicine and Rehabilitation
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
