The Impact of Composites with Silicate-Based Glasses and Gold Nanoparticles on Skin Wound Regeneration.

Sorin M Mârza,Lucian Baia,Emilia Licărete,Klara Magyari,Mirela Moldovan,Adrian Florin Gal,Flaviu Tăbăran,Sorina Suarasan,Andras Nagy,Sidonia Bogdan,Cosmin Peștean,Robert Cristian Purdoiu,Ionel Papuc

doi:10.3390/molecules26030620

Abstract

The silver content of the skin regeneration ointments can influence its regeneration process but in the meantime, it can take the benefit of the antibacterial properties of silver by avoiding the bacterial infection of an open wound. In the current study, the skin healing and regeneration capacity of bioactive glass with spherical gold nanocages (BGAuIND) in the Vaseline ointments were evaluated in vivo comparing the bioactive glass (BG)-Vaseline and bioactive glass with spherical gold (BGAuSP)-Vaseline ointments. Spherical gold nanocages are stabilized with silver and as a consequence the BGAuIND exhibits great antibacterial activity. Histological examination of the cutaneous tissue performed on day 8 indicates a more advanced regeneration process in rats treated with BGAuSP-Vaseline. The histopathological examination also confirms the results obtained after 11 days post-intervention, when the skin is completely regenerated at rats treated with BGAuSP-Vaseline compared with the others groups where the healing was incomplete. This result is also confirmed by the macroscopic images of the evolution of wounds healing. As expected, the silver content influences the wound healing process but after two weeks, for all of the post-interventional trials from the groups of rats, the skin healing was completely.

Highlights

Skin wound healing is the imperfect reflection of human wound healing processes in animals used as an experimental model
The obtained spectra are comparable with the reported ones [12]: the spectrum of bioactive glass (BG) has an electronic absorption band at 270 and 300 nm assigned to the Si-O-(Si, Ca), P-O-(P-Ca) chemical bonds; the surface plasmon resonance bands of BG-AuSP at 533 nm show the presence of nanoparticle with diameters
The BGAuIND with antibacterial effect was introduced into Vaseline ointments and was investigated in vivo for the future skin regeneration trials comparing with the BGVaseline and BGAuSP-Vaseline ointments regeneration capacity

Summary

Introduction

Skin wound healing is the imperfect reflection of human wound healing processes in animals used as an experimental model. They remain essential biological tools in creating of new techniques and approaches in the field [1]. A viable skin regeneration strategy is to involve tissue engineering that requires the use of high-potential biomaterials to accelerate the angiogenesis process and to ensure better vascularity [3,5]. Biomaterials with gold nanoparticles (AuNPs) can accelerate wound healing through several mechanisms: by decreasing the septic phase of healing by an antioxidant activity [6], through epithelial and mesenchymal cells migration in the injured skin, through myofibroblasts distinction and speeding up the angiogenesis cycle [7]. The ideal diameter of AuNPs is between 25 and 100 nm [8,9]

Methods

Results

Conclusion