THE IMPACT OF COMBINED EXERCISE TRAINING ON THE DEVELOPMENT OF CARDIOVASCULAR AND NEUROIMMUNE COMPLICATIONS INDUCED BY FRUCTOSE OVERLOAD IN HYPERTENSIVE RATS

Abstract

The aim of the present study was to evaluate the impact of combined exercise training on the development of cardiovascular and neuroimmune complications induced by fructose overload in sedentary hypertensive (SH), SHR + fructose (HF) and SHR + fructose + training (Treadmill + ladder, 60 days, 40–60% of maximal capacity) (HFTC). The groups were divided into subgroups evaluated at 7, 15, 30 and 60 days (n=6/group/time). Fructose was offered in drinking water (10%). Metabolic, hemodynamic, autonomic, inflammatory, and oxidative stress parameters were evaluated. Regarding the metabolic profile, the HF group showed increase in white adipose tissue, reduction in insulin sensitivity (HF: 3.15±0.2 vs. H: 3.96±0,1 and HFTC: 4.32±0.2 %/min) and increased triglycerides (TG) when compared to H group at 60 days of protocol (HF:139±7 vs. H: 106±5 and HFTC:107±8 mg/dL); these changes were not observed in HFTC group. Fructose consumption (HF) induced a further increase in mean arterial pressure (MAP) at 30 and 60 days in SHR (HF‐30 days: 153±4 e HF‐60 days: 184±4 vs. H‐30 days: 141±3 e H‐60 days: 165±3 mmHg). Combined exercise training reduced MAP in 30 and 60 days of fructose overload in relation to the HF group (HFTC: 142±8 and 167±6 mmHg). There was a decrease on heart rate variability (VAR‐PI) in 7 days and 60 days in the HF group (10.5±1.3 and 40.81± 6.12 ms2) in relation to the H group (23±1.5 and 59.7±3.4 ms2). The HF group had a lower alpha index (spontaneous baroreflex) at 7 days compared to the H group (0.23±0.03 vs. 0.35±0.01 ms/mmHg), which was not observed in the HFTC group. There was an increase in IL‐6 and TNFα in cardiac tissue at 15, 30 and 60 days in the HF and HFTC groups compared to the H groups. The HFTC group presented higher IL‐10 values in the heart at 7 days compared to the HF group (28±1 vs. 16±1 pg/mg/prot). The HF group presented increase in cardiac lipoperoxidation at 30 and 60 days in relation to the H group. The HFTC group decreased lipoperoxidation compared to HF group at 60 days (1302±58 vs. 1956±215 cps/mg/prot) and there was also reduction in protein oxidation in this time. In addition, there was an increase in NADPH oxidase in the HF group at 60 days when compared to the H group and reduction in this parameter in the HFTC group in relation to the HF group at the same time (H: 0.18±0.02; HF: 0.46 ±0.04; HFTC 0.35 ±0.03 μm/mg/prot). The HFTC group increase glutathione redox balance in 7, 15, 30 and 60 days in relation to H and HF groups. These findings reinforce the role of the autonomic nervous system in the genesis of cardiometabolic dysfunctions and evidence an important role of combined exercise training in the prevention of these alterations.Support or Funding InformationFAPESP: 2015/11223‐6, CAPES‐PROSUP (1277269), UNINOVEThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.