The Impact of Cold Acclimatization on Antioxidant Enzyme Activity of Vicia sativa L.

Abstract

Vicia sativa L. is one of most significant forages all over the world, but yield is decreased by cold stress. This study aimed to investigate the cold stress mechanism of V. sativaunder tissue culture in response to some biochemical analysis. In this study, six V. sativacultivars (Tarım Beyazı, Ankara Moru, 24 Çilli,Kansur and Aygün) were carried out to determine cold resistance in vitroconditions. Doğu Beyazı cultivar was used as a cold resistant plant. For the cold acclimation, two weeks old seedlings were incubated in the test chamber set at 4°C for 14 days to induce cold stress. Leaf samples were obtained at 14 days after cold treatment for physiological analysis evaluation. Cold resistant cultivars were chosen using values of thermal degrees. Moreover, antioxidant enzyme activities were determined at cold acclimated and non-acclimated seedlings. Peroxidase enzyme activities gradually increased at cold acclimation compared to those seedlings at non-acclimated.The highest POX activity was found at Ankara Moru seedlings, while the lowest activity was found at Aygün seedlings. SOD and APX activities were detected inversely at cold acclimation compared to those seedlings at non-acclimated. ChlorophyII A, chlorophyII B and carotenoid values were also determined at cold acclimated seedlings. The highest activities and pigment values were detected at Tarım Beyazı seedlings. Whereas, the lowest activities were found at Ankara Moru and Aygün cultivars. Our results displayed that cold acclimation linked to SOD, APX and POX activities and pigment estimation at in vitro conditions.