Abstract
BackgroundMounting evidence suggests that cesarean delivery may have a long-lasting effect on infant health. But the underlying mechanisms remain unclear. This study aims to examine whether cesarean delivery on maternal request without any medical indications (CDMR) impacts DNA methylation status in the umbilical cord blood of the infant.MethodsA cross-sectional study was conducted in Shanghai, China. A total of 70 CDMR and 70 vaginal deliveries (VD) were recruited in 2012. The cord blood DNA methylation status was measured in 30 CDMR and 30 VD newborns using Illumina Infinium Human Methylation 450 K BeadChip. To validate the results, the cord blood DNA methylation status was measured in another 40 CDMR and 40 VD newborns using targeted bisulfite sequencing assay. A total of 497 CpG sites from 40 genes were included in the analysis.ResultsA total of 165 differentially methylated positions (DMPs) exhibited differences in DNA methylation by 10% or more between the CDMR and VD groups, many of which were related to the development of the immune system. Based on the targeted bisulfite sequencing assay, 16 genes (16/22, 72.7%) had higher methylation level in the CDMR group than the VD group. Among them, 5 genes were related to the immune system. After considering the estimation of cell type proportions, there was few significant differences in DNA methylation between CDMR and VD groups.ConclusionsThe DMPs identified between CDMR and VD groups might be largely explained by the cell type proportions. Further studies are needed to examine DNA methylation in each cell type separately.
Highlights
Mounting evidence suggests that cesarean delivery may have a long-lasting effect on infant health
Our study aimed to investigate the association between cesarean delivery (CD) and DNA methylation status in the cord blood
Women who had Cesarean deliveries on maternal request (CDMR) and vaginal deliveries (VD) were matched on their age at delivery, prepregnancy body mass index (BMI), infant birthweight, and gestational weeks at delivery
Summary
Mounting evidence suggests that cesarean delivery may have a long-lasting effect on infant health. This study aims to examine whether cesarean delivery on maternal request without any medical indications (CDMR) impacts DNA methylation status in the umbilical cord blood of the infant. After labor for several hours, a totally dependent fetus must rely on his body systems to survive in the outside world. May be a physical process of birth and a physiological process to prepare the fetus to survive independently [1]. Pre-labor cesarean delivery (CD) interrupts this natural process and, might have important health. More and more evidence indicate that CD may have long-term impacts on maternal and child health.
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