The Impact of Black Seeds and Sidr Honey on paracetamol Induced Nephropathy in Adult Male Albino Rats, a Histological, Immunohistochemical & Ultrastructural study

Abstract

Abstract: Background: The kidneys play an important role in body homeostasis by conserving fluids and electrolytes and by disposing metabolic wastes. Kidney diseases are considered a public health issue worldwide. They contribute to approximately 850,000 deaths each year. Honey is a natural product which is widely used for its therapeutic effects. Black seeds have been used in traditional medicine for centuries as it is effective against many diseases. Aim of work: to study the possible protective effect of sidr honey and black seeds on experimentally induced nephropathy. Material & Methods: Thirty-six adult male albino rats were used in the present study. They were divided into six groups, 6 rats each. (GI): Control group, (GII) Paracetamol injected group by single intraperitoneal high dose of paracetamol (600 mg/kg) then sacrificed, (GIII): treated by Nigella Sativa and Sidr Honey followed by paracetamol injection (protective group), (GIV): Paracetamol injected group treated by Nigella Sativa and Sidr Honey (curative group). Blood Samples were collected, serum creatinine, albumin and blood urea nitrogen were estimated. Specimens taken from the left kidney were processed and examined by light microscope, and Specimens taken from the right kidney were processed and examined by electron microscope. Results: application of paracetamol caused destructive changes in the renal corpuscles and tubules, these changes were ameliorated by application of Sidr honey and black seeds in both protective and curative groups. Conclusion: Sidr honey and black seeds are effective agents in protection and treatment of kidney insults.