The impact of Arbuscular Mycorrhizal fungi on glomalin-related soil protein distribution and their relationship with soil properties of agroforestry plants in coal mine region of NorthTelangana

Abstract

The present study is to assess the effect of glomalin-related soil protein (GRSP) of four different coalmine overburden soils of threeplant species and evaluation of seed germination and abundance of AM fungal spores. Rhizosphere soil samples in four locations of the Telangana region has been examined to assess and exploit the distribution and diversity patterns of AMF. The rhizosphere soils of three forest tree species namely Albizia lebbeck, Acacia nilotica and Pongamia pinnata were collected for present investigations. Extracted both total glomalin (TG) and easily extractable glomalin (EEG) and scrutinized the above-mentioned rhizosphere soils. The effects of these EEG and TG on these plants collected from four different locations were also examined. The percentage of AM fungal root infection, spore populationwas examined to calculate the distribution of Arbuscular Mycorrhizal (AM) Fungi. 90 days older plants were usedfor observations. The AM fungal population was found to be highest in Kothagudem (GKOc) soils while least in Bhupalpally (KTK OC-III) soils. EEG and TG were highest in GKOC soils. The EEG was recorded lowest in the soils of Bhopalpally and TG was found to be least in Ellandhu soils. The EEG and TG were effective in inducing the seed germination and there was no co-relation between EEG and TG, but the percentage of glomalin was directly related to AMF population.