The impact of Ag nanoparticles on methane emission in two typical paddy soils

Abstract

Numerous applications of Ag nanoparticles (AgNPs) have increased the likelihood of their release and accumulation in agroecosystem. Thus far, few studies have evaluated the impacts of AgNPs to soil methane emissions and the microbial dynamics. In this study, microcosmic experiments were conducted to investigate the responses of methanogenic processes from two paddy soils (Cambisols and Ultisols) subjected to four AgNPs doses (0.1, 1, 10 and 50 mg/kg). The results showed that 0.1 and 1 mg/kg AgNPs had no significant effects on CH4 emissions, but 50 mg/kg AgNPs increased soil CH4 emissions in both paddy soils. The aggravation effect of AgNPs on CH4 emissions was more apparent in Ultisols compared to Cambisols paddy soils. Real-time PCR suggested that 50 mg/kg AgNPs significantly increased the ratio of methanogenic to bacterial gene for both paddy soils. Amplicon sequencing indicated that methanogenic community was clustered into a separate group after 50 mg/kg AgNPs exposure. Structural equation model illustrated that Methanosarcinales was both significantly responded to AgNPs in Cambisols and Ultisols soils; however, Methanocellales significantly responded to AgNPs only in Cambisols soils. Subsequently, uncontrolled use of AgNPs may account as an environmental risk due to the potentially increased soil CH4 emissions in paddy ecosystems.