Year-end Sale % OFF 
Abstract
This study aims at demonstrating the immunomodulatory property of jacaric acid, a conjugated linolenic acid (CLNA) isomer that is present in jacaranda seed oil, on murine peritoneal macrophages. Our results showed that jacaric acid exhibited no significant cytotoxicity on the thioglycollate-elicited murine peritoneal macrophages as revealed by the neutral red uptake assay, but markedly increased their cytostatic activity on the T-cell lymphoma MBL-2 cells as measured by the fluorometric CyQuant® NF Cell Proliferation Assay Kit. Flow cytometric analysis indicated that jacaric acid could enhance the endocytic activity of macrophages and elevated their intracellular production of superoxide anion. Moreover, jacaric acid-treated macrophages showed an increase in the production of nitric oxide which was accompanied by an increase in the expression level of inducible nitric oxide synthase protein. In addition, the secretion of several pro-inflammatory cytokines, including interferon-γ, interleukin-1β and tumor necrosis factor-α, was up-regulated. Collectively, our results indicated that the naturally-occurring CLNA isomer, jacaric acid, could exhibit immunomodulating activity on the murine peritoneal macrophages in vitro, suggesting that this CLNA isomer may act as an immunopotentiator which can be exploited for the treatment of some immunological disorders with minimal toxicity and fewer side effects.
Highlights
Conjugated fatty acids (CFA) are a mixture of the positional and geometric isomers of polyunsaturated fatty acids (PUFA) with conjugated double bonds, in which two carbon-carbon double bonds are separated solely by one carbon-carbon single bond [1]
Jacaric acid induced the cytostatic activity of murine peritoneal macrophages To demonstrate the immunomodulatory effects of jacaric acid on macrophages, the cytostatic activity of TG-induced murine peritoneal macrophages against MBL-2 tumor cells was evaluated by the CyQuant1 NF Cell Proliferation Assay Kit
The cytostatic effect was found to be more pronounced in the presence of LPS and it was found that the percentage of inhibition of MBL-2 cell proliferation reached 80% when the macrophages were pre-treated with 100 μM jacaric acid in the presence of LPS (Fig 2B)
Summary
Conjugated fatty acids (CFA) are a mixture of the positional and geometric isomers of polyunsaturated fatty acids (PUFA) with conjugated double bonds, in which two carbon-carbon double bonds are separated solely by one carbon-carbon single bond [1]. The most common and naturally-occurring CFA include conjugated linoleic acids (CLA) and conjugated linolenic acids (CLNA). Previous reports indicated that CLA can be found in meat and dairy products of ruminant animals [2] while CLNA occur in different plant seed oils [3]. CLA have been studied most extensively in view of their diverse metabolic and physiological effects [4,5] while CLNA have received increasing attention in recent years due to their relative abundance (up to 30– 70% of total lipids) in some plant seed oils [3,6]. Previous in vitro and in vivo studies have shown that CLNA exhibit pleiotropic physiological and pharmacological activities, including.
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
