The Immunoglobulin G Concentration in Colostrum and Blood Serum from Sarabi Calves in Iran

Abstract

The objective of the present study was to measure immunoglobulin G (IgG) levels in colostrum and blood serum of Sarabi calves as a genetic source of Iran. IgG levels determined in the first colostrum after calving by a SRID kit and also in the blood serum by ELISA and SRID. Twenty colostrum samples were taken from cows' immediately after parturition and stored at -20 °C until analysed. Venous blood samples for determination of plasma IgG concentration were obtained from 20 calves immediately before colostrum administered and after 24 h, 48 h and 8 days of birth. The maximum, minimum and mean (± SE) levels of IgG in the colostrum samples were 17000, 2000 and 8825±1206 mg/dl, respectively. The IgG levels (Mean± SE) in blood serum of calves were 451±17, 493 ±16, 508±26, and 503±24 mg/dl before colostrum intake, 24 h, 48 h, and 8 days after birth, respectively. The maximum concentrations of IgG in the blood serum (by ELISA) were 634, 631, 697 and 696 mg/dl in different time-points, respectively. IgG concentration in 4 calves that measured by SRID were 180, 1138, 1017, and 897 mg/dl in different time, respectively. The results showed that plasma IgG in calves was lower than 10 g/l, indicating existence of failure of passive transfer (FPT) in all the studied animals. Therefore, the Sarabi calves are prone to present clinical signs of FPT syndrome.