The Immunization of Protoscolices P29 DNA Vaccine on Experimental Cystic Echinococosis in Balb/c Mice.

Abstract

Cystic Echinococosis is one of the important parasitic diseases that is considered as a problem economics and health in many parts of the world. Many efforts have been performed for controlling the disease in the world. To reach a reliable vaccine against Cystic Echinococosis is one of the important duty of governments. Several antigen of hydatid cyst for vaccine candidate have been evaluated. In this study, P-29 antigen has been used for this purpose. E.g P29 antigen was cloned in Escherichia coli and transfected into the Chinese hamster ovary cell for antigen proliferation and used for vaccination in Balb/c mice. The recombinant antigen E.g-29 was shown using Western blot test. Two dilution of DNA vaccine (pCEgP-29) including 50µg/100µl and 100µg/100µl were prepared. Twenty four Balb/C male 6-8week mouse were divided in 4 groups. The groups were included in 2 vaccination groups (pcEg.P29 50µg/100µl and 100µg/100µl dilution) as immunized groups and 2 groups of plasmid and PBS as control. The mice were injected intramuscularly 3 times with 2weeks interval. After 3weeks from last injection, all groups were challenged intraperitonealy with 2000 protoscolices. After 5months, the mice were euthanized by ketamine/xylasine injection and number, size, and weight of cysts were recorded. Immunization rate was up to 93% in vaccinated group when compared with the control group. The results of this study showed that rEg.P29 could be considered as an effective vaccine for controlling of E. granulosus prevalence in intermediated host.