The immune response to Echinococcus granulosus: Sequential histological observations of lymphoreticular and connective tissues during early murine infection

Abstract

This paper describes the organ weight changes and the early (0 to 56 days) histological and ultrastructural changes in the lymphoid structures and injection sites after subcutaneous infection of Balb c mice with Echinococcus granulosus protoscolices (psc). Local lymph nodes enlarged markedly, reaching maximal weight 5 to 14 days after infection (pi). Expansion of the thymus-dependent areas of draining lymph nodes was first recognized at 3 days pi and reached a maximum between 14 and 21 days pi. Lymphoproliferation within B-cell-dependent areas was less extensive and of shorter duration. Comparable, but diminished, changes were seen in contralateral lymph nodes and spleen. Lymphoid cell depletion was observed in T-dependent areas of lymphoid organs by 56 days pi. Local inflammatory reactions to psc, at the site of injection, were intense, involving neutrophils, eosinophils, macrophages and mast cells. Viable psc were present until at least 6 weeks pi. The central role of the T-lymphocyte in recognition of, and response, to E. granulosus psc is discussed.