Abstract
Five bacterins consisting of formalin-killed, heat-killed, pH-lysed, Streptococcus bacterin (formalin-killed cells)-mixed, or ultraviolet (UV)-killed cells of Renibacterium salmoninarum were tested for the immune responses elicited in rainbow trout ( Oncorhynchus mykiss). Low levels of agglutinating antibody titres were formed in vaccinated fish and in some fish these were undetectable. Phagocytosis was significantly increased in vaccinated fish, except with the Streptococcus-mixed bacterin, and the chemiluminescent responses of leucocytes were stimulated only with the pH-lysed and UV-killed bacterins. However, no significant protection was observed in the fish when they were experimentally challenged.
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