Abstract
Objective To explore the regulation of flagella expression of Salmonella typhimurium in different growth phases and to investigate the relationship between flagella and immune response.Methods Flagella expression in the logarithmic phase and the stationary phase was measured by RT-PCR.Swimming motility was evaluated by motility assay.Macrophage pyroptosis rate was calculated by LDH assay and fluorescent dye staining.The expression of caspase-1 and IL-1β was detected by Western blotting assay.Results Flagella expression and swimming motility of Salmonella typhimurium in the logarithmic phase was higher than that in the stationary phase.Salmonella typhimurium in the logarithmic phase could induce expression of caspase-1 and IL-1β and trigger rapid macrophage pyroptosis,while salmonella in the stationary phase did not show this capability.Conclusion The high expression level of flagella in Salmonella typhimurium in the logarithmic phase could activate inflammasome and induce macrophage pyroptosis. Key words: Salmonella typhimurium; Flagella; Innate immunity; Inflammasome ; Pyroptosis
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