Abstract

Objective To understand the immune response changes in human leukocyte antigens (HLA) sensitized individuals in order to find out effective treatment for antibody-mediated rejection (AMR). Methods A HLA sensitized mouse model was established by skin graft using HLA-A2. 1 transgenic mice as donors and wild type mice as recipients. Different immune responses were studied in the sensitized recipients. Results Sensitizing mice with skin grafts from HLA-A2. 1 transgenic mice resulted in robust production of anti-HLA IgG, peak in 28 d[( 14. 10 ±0. 47) μg/L, enzyme linked immunosorbent assay (ELISA)], mainly IgG2a[(449. 4 ± 113.5) μg/L, LATM120], accompanied by changes in the constitution of different B and T lymphocytes in peripheral blood, spleen, and bone marrow, also accelerated rejection of a secondary skin allograft[2nd graft survival day (3.0 ± 0. 7 ) d vs 1 st graft survival day ( 10. 0 ± 1.3 ) d]. Sensitization of recepients with single HLA-A2 induced antibodies which had cross reaction with a panel of class Ⅰ HLA. These antibodies had cytotoxic effect targeting HLA-A2 expressing cells.Conclusion HLA-A2 sensitization resulted in abundant production of IgG2a accompanied by activation and proliferation of different B and T lymphocytes. These antibodies cross-reacted with a panel of class Ⅰ HLA, and may be the cause of AMR after allogenic skin graft. Key words: Skin graft; Immune response; Anti-HLA antibody

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