Abstract
Quantitative X-ray microanalysis of diffusible elements is commonly practised with frozen-dried cryosections. However the contents of matrix-free extracellular spaces cannot be preserved during freeze-drying; the elements in such spaces can be analysed quantitatively only in specimens in the frozen-hydrated state. One obstacle discouraging wider use of this approach is that the low image-contrast in frozen-hydrated sections makes it difficult to identify the fields one wants to analyse. Here we shall illustrate the problem and consider several bases for coping with it.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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