Abstract
» Amplifi cation of the SPI-sequence from a cDNA of Hevea brasiliensis » Identifi cation of the sequence in a pDrive vector system (EMBL accession number: HF937118) » Expression of the SPI variant in the pMAL system » Preparative isolation of a soluble, recombinant maltose-binding protein (MBP)-SPI fusion protein » Biotinylation of the SPI-hybrid and coupling onto StreptavidineImmunoCAPs (ThermoFisher Scientifi c, Uppsala , Sweden) » 21 sera of health care workers (HCW) with symptoms to natural rubber latex (NRL) were tested for SPI and all available single latex allergens
Highlights
Serine protease inhibitors (SPI) comprising 60-90 amino acid residues, are frequent in plants, and belong to the protein family PR-6
It has recently been shown that a SPI-variant of wheat acts as an important allergen in baker’s asthma but not in persons suffering from wheatinduced food allergy
The aim of this study was to elucidate the role of a recombinant SPI-variant of Hevea brasiliensis for occupational latex allergy
Summary
Serine protease inhibitors (SPI) comprising 60-90 amino acid residues, are frequent in plants, and belong to the protein family PR-6. It has recently been shown that a SPI-variant of wheat acts as an important allergen in baker’s asthma but not in persons suffering from wheatinduced food allergy. The aim of this study was to elucidate the role of a recombinant SPI-variant of Hevea brasiliensis for occupational latex allergy
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