Abstract
The preparations of chymotrypsin C (EC 3.4.21.2) and an acidic endopeptidase from porcine pancreas have been repeated using published procedures. The acidic endopeptidase showed lower activity than chymotrypsin C in all comparative experiments, but it was possible to precipitate a fraction from the acidic endopeptidase preparation which contained all the protein and was identical with chymotrypsin C. It is concluded that the acidic endopeptidase is identical with chymotrypsin C but it is contaminated by an inert non-protein material to which it is firmly bound. The formation of a precipitate, at low ionic strength, from mixtures of chymotrypsin C and elastase (EC 3.4.21.11) is independent of the availability of the active site of either enzyme.
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