Abstract
The study was aimed at identification, production and characterization of nattokinase, bacteriocin from bacterial species. Nattokinase and bacteriocins finds a wide range of applications in Pharmaceutical industry, health care and medicine. Nattokinase is a highly active fibrinolytic enzyme secreted by Bacillus subtilis and bacteriocins are proteinaceous toxins produced by Lactobacillus to inhibit the growth of closely related bacterial strains. Bacillus subtilis and Lactobacillus isolates shown positive results to microscopic, biochemical analysis. The nattokinase and bacteriocins were produced by optimizing the media. The enzymes were purified by ammonium sulfate precipitation and HPLC. The enzyme activity for nattokinase was found at 7 mg/ml, pH 8.0 and temperature 48 ºC and the enzyme activity for bacteriocin was found at 3.9 mg/ml, pH 6.5 and temperature 30 °C. Bacteriocins from Lactobacillus showed good antagonistic activity against pathogenic bacteria. Nattokinase from Bacillus subtilis played a significant role in thrombolytic and anti-coagulation at in vitro. The results indicated that the pure enzyme has a potential in dissolving blood clot.
Highlights
Nattokinase is a fibrinolytic enzyme that belongs to the second wide family of serine protease
The fibrinolytic activity of Nattokinase can be retained in the blood for more than 3 hours, which makes it probable for clinical use and it is widely considered as a promising oral medicine for thrombolytic therapy (Vignesh H et al, 2014)
For Bacillus subtilis bacterial colonies were produced as the result of pour plate method which was done on nutrient agar plates which were incubated at 37 °C overnight
Summary
Nattokinase is a fibrinolytic enzyme that belongs to the second wide family of serine protease. It hydrolyses fibrin and is the major protein constituent of blood clots, an insoluble white protein formed by the conversion of fibrinogen through thrombin. Nattokinases was initially discovered and extracted from Japanese conventional fermented soybean foods that were of certain importance due to their efficient biological thrombolysis of fibrin and clots of blood in blood vessels (Sumaya Ali Hmood et al, 2016). The fibrinolytic activity of Nattokinase can be retained in the blood for more than 3 hours, which makes it probable for clinical use and it is widely considered as a promising oral medicine for thrombolytic therapy (Vignesh H et al, 2014).
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