The Identification of Variation Sequences from Ovulation Rate Gene as Genetic Candidate for Twin Birth Markers in East Java Local Cows

Abstract

The level of public consumption of beef continues to increase along with public awareness of the importance of animal protein needs. This increase continues every year. However, this is not balanced with an adequate increase in the amount of production, so the right solution is needed to overcome it. The purpose of this study was to identify variations and expressions of gene ovulation rates as genetic candidates for multiple birth markers in East Java-local cows. The identification was carried out in several stages including DNA isolation, PCR amplification, electrophoresis, sequencing, and MEGA 7 analysis. The results obtained from the isolation stage were then analyzed using MEGA 7. This analysis is a computer application program designed to compare and analyze homologous gene sequences. The program is also equipped with the results in the form of phylogenetic trees and evolutionary distance matrices. The results gained showed the analysis of sequencing results using MEGA7 software; there were variations in the sequence of nucleotide bases in the four samples. A number of these differences indicated that the four samples had differences, but there was still kinship. The analysis results showed that the primary ovulation rate gene was one of the marker genes that could be used in the detection of multiple births in local cattle in East Java.