The identification of infective filarial larvae in mosquitoes: with a note on the species found in "wild" mosquitoes on the Kenya coast.

Abstract

1. At least twenty-two species of filarial worms are known to develop in mosquitoes. Filarial larvae of non-human origin must often be present in mosquitoes dissected during filariasis surveys yet the larvae seen are commonly assumed to be of human origin.2. On Pate island off the Kenya coast where bancroftian filariasis is prevalent, several distinct species of infective larvae were found in mosquitoes both in the houses and in the bush. Seven species of filarial worms were found in the local animals and a collection of known infective larvae was made by feeding mosquitoes on these different infections. A study of the material in this “reference collection” showed that many of the larvae in “wild” mosquitoes could be identified with confidence.3. A technique has been developed for the dissection of preserved and stained mosquitoes; this method has many advantages and can be used in routine filariasis surveys.4. The characters which were most useful in differentiating the infective larvae were: the length; the position of the anus; and the shape of the caudal extremity. Eight species of infective larvae were available for study, these are described and illustrated: W. bancrofti, W. malayi, W. pahangi, W. patei, D. corynodes, D. repens, D. immitis and S. equina. The literature dealing with a further fourteen species is reviewed.5. A simple key has been produced to help identify the species of infective larvae found in mosquitoes.6. On the Kenya coast W. bancrofti infective larvae have been found only in C. fatigans, A. gambiae and A. funestus. Infective larvae of animal origin which might easily be mistaken for those of W. bancrofti have been found in Ae. pembaensis, Ae. aegypti, M. uniformis and M. africanus.7. Formulae for transmission indices based on infection rates in insect vectors are of little value unless there is confidence in specific identification.8. A study of the infective larvae in Simulium neavei from an onchocerciasis area in Uganda has shown that not all the larvae are of human origin. A closer examination of the morphology of infective larvae in the vectors of filarial parasites of man in other areas will almost certainly show that the interpretation of infection rates may be complicated by the presence of infections of nonhuman origin.9. Feeding experiments were carried out with Aedes pembaensis and Ac. aegypti on a donkey infected with Setaria equina; both of these mosquitoes proved to be suitable vectors of this filarial worm.