Abstract
Ethidium bromide (EtBr) is widely used in most laboratories to detect nucleic acids in gel electrophoresis applications. It is a well-known carcinogenic and mutagenic agent, which can affect biotic components of the place in which it is disposed. Usually the gel-waste is either buried in the ground or incinerated, whereas the liquid waste is disposed of down the sink following the recommended methods of treatment. The recommended methods do not involve biological potential, but rather make use of chemicals, which may further deteriorate soil and water quality. The present study identifies and characterizes the EtBr-degrading bacterial isolates BR3 and BR4. A bibliographic review of the risk status of using these isolates for the treatment of lab waste in laboratory settings is also presented. BR3 was identified as Proteus terrae N5/687 (LN680103) and BR4 as Morganella morganii subsp. morganii ATCC 25830 (AJ301681) with 99.9% and 99.48% similarity, respectively, using an EzBioCloud microbial identifier. The literature revealed the bacterium Proteus terrae as a non-pathogenic and natural microflora of humans, but Morganella morganii as an opportunistic pathogen. These organisms belong to risk group II. Screening the sensitivity of these isolates to antibiotics revealed a sufficient number of antibiotics, which can be used to control them, if required. BR3 and BR4 exhibited resistance to individual antibiotics, ampicillin and vancomycin, whereas only BR3 was resistant to tetracycline. The current investigation, along with earlier reported work on these isolates, identifies BR3 as a useful isolate in the industrial application for the degradation of EtBr. Identical and related microorganisms, which are available in the culture collection repositories, can also be explored for such potential to formulate a microbial consortium for the bioremediation of ethidium bromide prior to its disposal.
Highlights
Nowadays, molecular biology has become a routine field of research in which ethidium bromide is used as a staining dye for nucleic acid visualization
The nucleotide sequence specific to the 16S-rRNA gene of isolates was uploaded in the identification module of EzBioCloud (CJ Bioscience, Inc., Seoul, Korea) and the isolates with their respective type strains were identified on the basis of maximum similarity
No study reported the antibiotic profile of P. terrae (BR3) M. morganii (BR4) from laboratory gel electrophoresis BioTech 2022, 3, x FOR PEER REVIEWwaste
Summary
Molecular biology has become a routine field of research in which ethidium bromide is used as a staining dye for nucleic acid visualization. There are a number of ways these wastes can be treated or decontaminated, including treatment with bleach, incineration, processing of the solution through Rohm and Haas Amberlite XAD-16 resin, Fenton-like reaction using MNCs (magnetic nanocatalysts) and other methods and products [4,5,6,7,8,9]. Some of these methods used agents, such as sodium nitrite and hypophosphorous acids, which are still harmful [4,10]. No toxic impact of EtBr accumulation or EtBr biotransformation was reported [13]
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