Abstract
1.1. Cell-free preparations from a strain of Bacillus cereus isolated in our laboratory release orthophosphate (Pi) from 2-aminoethylphosphonate (AEP). The overall reaction is dependent on the presence of pyridoxal phosphate and pyruvate in the reaction mixture.2.2. An intermediate has been isolated from the reaction mixture and identified as 2-phosphonoacetaldehyde on a number of criteria, including comparison with the authentic compound.3.3. Synthetic 2-phosphonoacetaldehyde is degraded by the cell-free preparations to acetaldehyde and Pi. This reaction is inhibited by orthosphosphite, and is not dependent on the presence of pyridoxal phosphate and pyruvate.4.4. This work establishes the following pathway for the breakdown of AEP in B. cereus:
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