THE IDENTIFICATION AND STUDY OF HERBAL MEDICINE (JAMU) FRAGMENTATION PATTERNS CONFIRMED ACETAMINOPHEN BY USING LIQUID CHROMATOGRAPHY MASS SPECTROSCOPY (LCMS)

Moh Adam Mustapa,Muhamad Handoyo Sahumena,Endah Nurrohwinta Djuwarno,Muhammad Taupik

doi:10.55047/comorbid.v1i1.38

Abstract

 In the last twenty years, many of jamu or herbal medicine were adulterated with chemical drugs. One of the chemical drugs that is commonly added to herbal medicine is acetaminophen. Acetaminophen is generally considered as an analgesic-antipyretic drug. The sample of this study consisted of 6 samples of jamu or herbal medicine with different brands. Qualitative analysis was conducted by TLC method using the mobile phase n-hexane: ethyl acetate (1:1), it was obtained three positive samples containing acetaminophen, namely sample D, E, and F. The three positive samples containing acetaminophen were calculated using the LCMS method. Quantitative analysis was conducted with LCMS using a reversed-phase with a mobile phase of acetonitrile: water (15%>: 85%> v/v) at a flow rate of 0.2 mL/min and an injection volume of 5 gL, it was obtained the levels of acetaminophen in sample D as much as 0.16 g/2 g of sample, in sample E of 0.63 g/7 g of sample, and in sample F of 0.56 g/7 g of sample. Although the content of acetaminophen in herbal medicine is relatively small, but according to the Regulation of the Minister of Health of Indonesia No. 007 of 2012, traditional medicine or herbal medicine should not contain synthetic chemicals.

Highlights

The use of traditional medicine, such as jamu or herbal medicine in both medical and economic perspective tends to increase the growth of the herbal industry both on a factory and home industry scale
This Liquid Chromatography Mass Spectroscopy (LCMS) method can be used to determine complex compounds contained in herbs in comparison to the acetaminophen
This Thin Layer Chromatography (TLC) method is an initial verification process to sort out samples expected to contain acetaminophen

Summary

INTRODUCTION

The use of traditional medicine, such as jamu or herbal medicine in both medical and economic perspective tends to increase the growth of the herbal industry both on a factory and home industry scale. This development has an impact on the herbal medicine trade competition. One solution to overcome the problems of the identification process is to use the LCMS method (Liquid Chromatography Mass Spectroscopy). This method combines two methods in one analysis system. LCMS method is considered a fast, simple and cost-effective method

RESEARCH METHOD Tool

AND DISCUSSION

Conclusion

Sample D 2 Sample E 3 Sample F

Results

CONCLUSION