Abstract
The first phase of the ESRF beamline ID23 to be constructed was ID23-1, a tunable MAD-capable beamline which opened to users in early 2004. The second phase of the beamline to be constructed is ID23-2, a monochromatic microfocus beamline dedicated to macromolecular crystallography experiments. Beamline ID23-2 makes use of well characterized optical elements: a single-bounce silicon (111) monochromator and two mirrors in Kirkpatrick-Baez geometry to focus the X-ray beam. A major design goal of the ID23-2 beamline is to provide a reliable, easy-to-use and routine microfocus beam. ID23-2 started operation in November 2005, as the first beamline dedicated to microfocus macromolecular crystallography. The beamline has taken the standard automated ESRF macromolecular crystallography environment (both hardware and software), allowing users of ID23-2 to be rapidly familiar with the microfocus environment. This paper describes the beamline design, the special considerations taken into account given the microfocus beam, and summarizes the results of the first years of the beamline operation.
Highlights
The average size of crystalline samples brought to macromolecular crystallography (MX) synchrotron beamlines has decreased markedly over recent years
Recently Moukhametzianov et al (2008), using ID13, published results demonstrating the feasibility of producing good quality diffraction using a 1 mmdiameter X-ray beam and a xylanase crystal
This paper describes beamline ID23-2 which is the first dedicated and highly automated high-throughput MX microfocus beamline
Summary
The average size of crystalline samples brought to macromolecular crystallography (MX) synchrotron beamlines has decreased markedly over recent years. The Diamond I24 dedicated microfocus MAD beamline is in operation accepting users since late 2008 In contrast to these microfocus beams, which provide very high flux densities, Sanishvili et al (2008) have implemented a mini-beam apparatus capable of collimating a beam to 5– 10 mm in combination with a 125 mm  25 mm focus at the Advanced Photon Source beamline 23ID-B, with data being successfully collected from a sample of 10 mm  10 mm  10 mm in size. This paper describes beamline ID23-2 which is the first dedicated and highly automated high-throughput MX microfocus beamline
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
