The hypoglycemic and hypolipemic potentials of Moringa oleifera leaf polysaccharide and polysaccharide-flavonoid complex

Abstract

In this study, Moringa oleifera leaf (MOL) flavonoids (MOLF) with strong α-glucosidase inhibitory activity and MOL polysaccharides (MOLP) with strong cholic acid-binding capacity were efficiently prepared by two-stage extraction method and mixed in a certain proportion for development of MOL highly-processed products with hypoglycemic and hypolipemic potentials. Quercetin-3-O-glucoside (6.86%) and kaempferol-3-O-glucoside (4.02%) were identified as the main components of MOLF. MOLP constructed by galactose, arabinose, rhamnose and galacturonic acid possessed the strongest effects on delaying glucose diffusion and dialysis, delaying starch digestion, binding bile acids and inhibiting cholesterol micelle solubility, being the best MOL highly-processed products for regulating carbohydrate and lipid digestion and absorption. MOLF and MOLP had synergistic effect on delaying glucose diffusion and dialysis, delaying starch digestion and binding bile acids, while MOLF impaired the inhibitory effect of MOLP on cholesterol micelle solubility. Compared with MOL primary-processed products including MOL powder and de-phenolic MOL powder, MOL highly-processed products including MOLP and MOLF-MOLP complex possessed stronger hypoglycemic/hypolipemic potentials.