Abstract
The Hydrolysis of Long-chain Fatty Acid Esters of Cholesterol with Rat Liver Enzymes
Highlights
The present study reports the hydrolysis of long-chain fatty acid esters of cholesterol with enzymes present in the microsomal and soluble fractions of rat liver
Tripalmitin hydrolysis (15% of 20 mpmoles in 1 hour) slightly exceeded that of cholesteryl oleate and linoleate at similar concentrations. These experiments show that rat liver contains enzymes that hydrolyze the long-chain fatty acid esters of cholesterol, and that the bulk of hydrolytic activity is found in the soluble fraction of the liver homogenate
It is clear that the lesser activity of the microsomes alone was not, due to the presence of a relatively large pool of endogenous substrate in the microsomes, and that most of the hydrolytic activity of the S-10 was contributed by the soluble fraction
Summary
Enzyme Preparations-Fed male Sprague-Dawley rats, weighing 175 to 225 g, were killed by decapitation. After removal of nuclei and cell debris by centrifugation at 2,000 X g for 30 minutes, the supernatant fraction (S-2) was centrifuged at lO,-. T Present address: Department of Medicine, Beth Israel Hospital and Harvard Medical School, Boston, Massachusetts. Microsomes were sedimented from the 10,000 X g supernatant fraction (S-10) by centrifugation at 104,000 X g for 1 hour. The soluble supernatant fraction (S-104) was removed, and the microsomes were washed by suspension in a large volume of buffer followed by centrifugation as before. No efforts were made to remove the layer of free-floating fat from the S-104. In later experiments this layer was removed and discarded, and the remaining supernatant called the “defatted”
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
