The human ribosomal protein S6 gene: isolation, primary structure and location in chromosome 9

Abstract

Using PCR cloning we isolated the first intron of the human ribosomal protein S6 gene ( hRPS6). By screening the human HeLa cell cDNA library in λZAPII vector (Stratagene, La Jolla, CA), we identified and sequenced a partially spliced pre mRNA copy of hRPS6. The complete hRPS6 gene was isolated from a λDASH library with an intron-specific probe. The gene and flanking regions were sequenced, and the mRNA 5′ end was mapped by primer extension experiments. The hRPS6 gene has 6 exons and 5 introns and is 3.6 kb long. Using intron-specific primers in PCR and a panel of human-hamster cell lines we localized the hRPS6 gene in human chromosome 9.