Abstract
Human periapical cystic tissue analyzed in the study was obtained from healthy volunteers donors. The cysts received several washes in sterile phosphatase buffer saline (PBS, Invitrogen) containing antibiotics. Cells from human periapical cystic tissue were isolated and characterized by flow cytofluorimetry: these cells expressed mesenchymal stem-like properties, so the authors termed them “human Periapical CystMesenchymal Stem Cells” (hPCy-MSCs). No study has up to now evaluated the possible existence of MSCs in human periapical cysts. In this research, the authors have demonstrated that cells isolated from human periapical inflammatory cysts display MSC-like properties, as evidenced by the expression of the MSC markers CD13 + , CD29
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