Abstract
The existence of β-adrenergic receptors was demonstrated on whole A431 cells as well as A431 membrane preparations by means of binding assays using the hydrophobic 1-[ 3H]dihydroalprenolol and the hydrophilic antagonist [ 3H]CGP-12,177 as β-adrenergic ligands. Binding was stereospecific. The receptors, as shown by competition studies, proved to be of the β 2-subtype and appeared functional in the stimulation of adenylate cyclase. The number of receptors per cell and the yield of receptor sites/mg membrane protein render the A431 cell a useful tool for the study of human β-adrenergic receptors.
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