The Human Bradykinin B2 Receptor Gene: Full-Length cDNA, Genomic Organization and Identification of the Regulatory Region

Abstract

Hybridization of Northern blots with a probe corresponding to the coding region of the bradykinin B2 receptor revealed a single transcript of approximately 4.0 kb. Using the same probe, we detected a 2.6 kb human cDNA clone that overlapped 103 bp with the 3′ end of the known coding region and contained a classical polyadenylation site and a polyA tail. The gene for the human B2 receptor was isolated from a human placenta genomic library. Analysis of several genomic λ clones indicated that the B2 receptor gene is organized in three exons. Genomic Southern analysis revealed the B2 receptor to be encoded by a single copy gene. In situ hybridization showed that the gene is located on chromosome 14q32. By testing different putative regulatory fragments in a luciferase reporter assay, a gene activating function of the 5′ upstream region of exon 1 was demonstrated.