Abstract
Simple SummaryConsidering recent research, it was established that the best experimental models to conserve biological features of human tumors and to predict individual clinical treatment success are patient-derived xenografts (PDX). Their recognized and growing importance for translational research, especially for late-stage preclinical testing of novel therapeutics, necessitates a high number of well-defined PDX models from individual patients’ tumors. The starting platform for the Hansestadt Rostock colorectal cancer (HROC)-Xenobank was the assortment of colorectal tumor and normal tissue samples from patients stored in our university biobank.Based on our research group’s large biobank of colorectal cancers (CRC), we here describe the ongoing activity of establishing a high quality assured PDX biobank for more than 100 individual CRC cases. This includes sufficient numbers of vitally frozen (n > 30 aliquots) and snap frozen (n > 5) backups, “ready to use”. Additionally, PDX tumor pieces were paraffin embedded. At the current time, we have completed 125 cases. This resource allows histopathological examinations, molecular characterizations, and gene expression analysis. Due to its size, different issues of interest can be addressed. Most importantly, the application of low-passage, cryopreserved, and well-characterized PDX for in vivo studies guarantees the reliability of results due to the largely preserved tumor microenvironment. All cases described were molecularly subtyped and genetic identity, in comparison to the original tumor tissue, was confirmed by fingerprint analysis. The latter excludes ambiguity errors between the PDX and the original patient tumor. A cancer hot spot mutation analysis was performed for n = 113 of the 125 cases entities. All relevant CRC molecular subtypes identified so far are represented in the Hansestadt Rostock CRC (HROC)-Xenobank. Notably, all models are available for cooperative research approaches.
Highlights
Despite early diagnostic options and improved treatment, colorectal cancer (CRC)is still one of the leading causes of cancer-related deaths worldwide [1]
We used our large collection of patient material, which included matching tumor and normal epithelial tissue, as a starting platform to establish a high number of individual patient-derived xenograft (PDX) models
The present study focuses on 125 of these cases, which have been selected according to the following criteria: (I) enduring growth in immunodeficient mice and (II) storing sufficient quantities of PDX tissues with (III) adequate quality
Summary
Despite early diagnostic options and improved treatment, colorectal cancer (CRC)is still one of the leading causes of cancer-related deaths worldwide [1]. The possibility of precisely predicting individual clinical treatment success, especially for the late-stage preclinical testing of novel therapeutics, implies a clear exigency for more academically run PDX-biobanks, containing large numbers of individual tumors [2,3,5,6] Inspired by this notion, we used our large collection of patient material, which included matching tumor and normal epithelial tissue, as a starting platform to establish a high number of individual PDX models. We used our large collection of patient material, which included matching tumor and normal epithelial tissue, as a starting platform to establish a high number of individual PDX models This resulted in a quality assured PDX biobank containing more than 100 individual CRC cases and encompassing all specific CRC molecular subtypes. Omics data from both the PDX model and the original patient tumor could, on the one hand, accelerate the entry of novel drugs into the clinic, and, on the other hand, such paired data sets would facilitate the identification and validation of predictive biomarkers [2]
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