Abstract
The application of a practical approach for the optimization of the separation of a mixture of triazine and substituted urea herbicides and a mixture of organophosphorous insecticides is presented. The separation is achieved with reversed-phase HPLC and acetonitrile/water mobile phase mixtures. The separation method is based on the simultaneous optimization of temperature (T) and gradient steepness (b) as a means of varying selectivity and achieving an acceptable resolution. The effect of flow rate is also examined. Triazine and substituted urea herbicides exhibit greater changes in resolution than organophosphorous insecticides with variation in T and b. This is attributed to the more diverse and polar molecular nature of the herbicide mixture. Flow rate is used as an additional parameter that could facilitate resolution. The overall separation is achieved within a few experiments.
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