The histopathological changes induced by intra peritoneal administration of cerium oxide on mice brain tissue

Abstract

The use of nanoparticles (NPs) and nanoparticle- based materials raises many concerns about their impact on health related to its many applications in diverse fields, including petroleum refining, polishing agents, and coatings, as well as the toxicity of these NPs in biological/ physiological environment is a major concern. The study conducted at college of nursing – university of Basrah –Iraq,and it aimed to investigated the effect of cerium oxide nanoparticles on mice brain tissues after 30 days of intra-peritoneal I/P injection of cerium oxide nanoparticles . White laboratory mice of Mus musculus L. strain BALB/C that were supplied from (Drug Control Center - College of Science, Thi -Qar University). The mice were placed under controlled conditions of 20-25 degrees and a 12-hour light cycle. And 12 hours of darkness in plastic cages of standard sizes (30×12×11) cm, the first group injected laboratory mice with 1 ml of normal saline physiological solution as a control group. The second treatment group. Laboratory mice were injected with 1 ml of cerium dioxide at a concentration of 3.75 µl of the raw material to 996.25 µl of physiological solution. By one injection between one day and another for a period of 30 days to investigate the effects of I/P administration of cerium oxide Nano- particles on mice brain tissue after 30 day. Mice were killed and brain isolated to prepare for the histological examination which showed that cerium oxide injected for 30 day induce pathological alteration in brain tissue especially on white matter , neurofibers and glial cells. Brain of mice show degeneration of white matter, ,necrosis of glial cell aggregation of glial cell , The histopathological examination investigate that intra-peritoneal injection in mice for thirty day induce histopathological adverse changes in mice brain tissue.