The histochemical localization of cytochrome oxidase in the retina and lateral geniculate nucleus of the ferret, cat, and monkey, with particular reference to retinal mosaics and ON/OFF-center visual channels

Abstract

The histochemical localization of cytochrome oxidase within the normal retina and lateral geniculate nucleus (LGN) of cats, ferrets, and monkeys revealed that distinct layers, types of cells, and portions of neurons are more intensely stained than others. The dark staining of photoreceptor inner segments and cone pedicles and the light staining of photoreceptor outer segments, somata, and rod spherules demonstrates that different segments of the same cell may have disparate but distinct levels of oxidative enzyme activity. In tangential sections of retina, regular mosaic arrays were evident for each of several darkly reactive retinal components, such as cone inner segments, cone pedicles, and horizontal cells. In the cat and ferret, regular mosaic arrays were also formed by metabolically distinguishable populations of ganglion cells. Ia and IIa ganglion cells (OFF-; Nelson, R., E. V. Famiglietti, Jr., and H. Kolb (1978) J. Neurophysiol. 41: 472-483) were more darkly reactive than the other classes. The darker staining of sublamina a of the inner plexiform layer (OFF-; Famiglietti, E. V., Jr., and H. Kolb (1976) Science 194: 193-195) in the cat and ferret retina, as well as sublamina A' and A1' of the ferret LGN (OFF-; Stryker, M.P., and K.R. Zahs (1983a) J. Neurosci. 3: 1943-1951) suggest that, under typical rearing conditions, the OFF-channels may be metabolically more active than the ON-channels in these species. In Macaca and Saimiri, darker staining was observed in sublamina b of the inner plexiform layer (ON-; Famiglietti, E.V., Jr., and H. Kolb (1976) Science 194: 193-195) and laminae 1, 2, and 6 of the LGN, implying that, under similar rearing conditions, a different pattern is observed. The dark staining of many large retinal ganglion cells, as well as most of the larger LGN neurons (presumed Y/Y-like), in all species studied is evidence that the Y/Y-like pathway is also highly active.