Abstract
A histochemical technique specific for zinc was achieved by means of a dithizone complex-forming solution. With this solution, zinc in prostate and stomach sections could be clearly differentiated from other metallic constituents. Dithizone in aqueous-acetone solution alone demonstrated non-specific staining in stomach, prostate, pancreas, and erythrocytes and fat of dog, man, rabbit, and rat. The staining of metals as contrasted with fat concentration of the dye was shown by loss of stain in tissues previously chelated with ethylenediaminetetracetic acid. Staining of phagocytized zinc oxide in macrophages indicated penetration of the cellular membranes by dithizone. Zinc acetate given intravenously to rabbits showed a marked concentration of zinc in the epithelial cells of small and large intestine. This experiment indicated that the intestines, particularly the small intestine, were a possible excretory route for zinc.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
