The heterogenity of anti-Mullerian hormone depletion models across assays in 12917 women

Abstract

OBJECTIVE: A regular Anti-M€ullerian hormone diagnosis obtained with different kits is still an open problem to opinion makers in the field of reproductive medicine. As far now the literature points out that the profile of the rate of AMH change varies for available assays. Thus, predominately important is to measure the heterogeneity among kits. DESIGN: The study was prospectively conducted on 12917 women (aged between 20 and 50 years) examined with four AMH assays. AMH measurements were performed during the time period between January 2007 and December 2012 in Invicta Fertility Centre, Poland. MATERIALS ANDMETHODS: 12917 women were examined with four AMH assays: Immunotech I generation kit (ImI 4016 samples), Beckman Coulter II generation kit RUO (BCII RUO 3430 samples), Beckman Coulter II generation kit with IVD certificate (BCII IVD 830 samples) and Ansh Labs I generation kit (AnshLabs 4641 samples). RESULTS: The data revealed the lack of parallelism in AMH depletion between assays at P< .05, when pairwise comparisons of assays were performed. Also the distribution of AMH concentration in quartiles between kits was statistically different (P< .05). Consequently, calculations proved an interaction between age variable and assays.Based on evidences we concluded that heterogeneity in the rate of AMH change exists and the central tendency for AMH levels across assays is substantially different. Finally, AMH diagnosis in women is dependent on an assay-age effect. CONCLUSIONS: We claim future studies should endeavour to create a profiles for heterogeneity and homogeneity of slopes between kits along age periods. This will give a certain medical aid to decision makers (clinicians), who perform opinions on infertility problem in patients. Those gynaecologists concerned with programming options for fertility preservation might benefit as well.