The Heterogeneity of Porcine 32,000 MrInhibin α-Subunit: A Gel Electrophoresis and Immunoblot Study*

Abstract

Porcine 32,000 Mr inhibin is a glycoprotein with one asparagine-linked glycosylation site on the alpha-subunit. The presence of carbohydrate on the alpha-subunit was visualized by periodate-Schiff (PAS) staining. This stain for carbohydrate also verified that the beta-subunit of 32,000 Mr porcine inhibin does not contain carbohydrate. When analyzed by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (1D-PAGE) under reducing conditions, the inhibin alpha-subunit consistently existed as a doublet, and by the PAS stain, both bands of the doublet were glycosylated. Analysis by two-dimensional (2D) PAGE further revealed the presence of charge isoforms of the alpha-subunit. The alpha-subunit of inhibin could be deglycosylated by N-glycanase, but not by endoglycosidase F, endoglycosidase D, or endoglycosidase H. When the N-glycanase-treated inhibin was analyzed by either 1D-PAGE or 2D-PAGE, the molecular size of the alpha-subunit was reduced by 3500 Mr. Each doublet band observed with reducing conditions in 1D-PAGE or 2D-PAGE for the alpha-subunit became a single band (spot) in the deglycosylated alpha-subunit. However, the charge heterogeneity detected by 2D-PAGE was retained, indicating that only a portion of this heterogeneity is attributable to the carbohydrate moiety. The in vitro biological activity of the deglycosylated inhibin was not different from the control sample. The composition of the carbohydrate in inhibin was investigated with the Dionex carbohydrate analyzer. Inhibin contains fucose, glucosamine, galactose, mannose, and glucose. Colorimetric analysis revealed the presence of sialic acid. Taken together, this implies some aspect of the peptide portion of the molecule is involved in charge heterogeneity. Inhibin may have an unusual carbohydrate component, as evidenced by the detection of glucose in inhibin samples. The absence of glucose in the carbohydrate moiety of another glycoprotein fraction that accompanied the inhibin through all the same fractionation procedures argues against the artifactual introduction of glucose in the fractionation medium per se.