The heterogeneity of fibroblasts in laryngotracheal stenosis and skin hypertrophic scar in pediatric patients

Abstract

ObjectivesTo investigate the heterogeneity between the laryngotracheal stenosis and hypertrophic scar derived fibroblasts. MethodsHuman laryngotracheal stenosis (LTS) and skin hypertrophic scar (HTS) specimens were obtained during the tracheal resection and T-shaped tracheal stent implantation surgery. Fibroblasts were isolated and cultured. Cell proliferation and migration were analyzed by cell count, EdU proliferation and wound-healing assays. The expressions of COL1a1, α-SMA, TGF-β1 signaling pathway, chemokines and receptors were analyzed by qRT-PCR, western blotting, and immunohistochemistry. ResultsCell proliferation and migration of LTS derived fibroblasts were significantly faster than HTS fibroblasts, with no significant difference of the percentage of apoptotic cells. COL1a1, α-SMA, and Integrins were down-regulated in LTS fibroblasts, but TGFB1 and chemokine receptor CXCR7 were up-regulated in LTS fibroblasts. However, the expressions of SMAD4 and phospho-SMAD2/3 were not significantly different. ConclusionsHuman LTS and HTS derived fibroblasts differ in cell proliferation and migration. Different expressions of COL1a1, α-SMA, and CXCR7 were found between the two fibroblasts.