Abstract
The nondialyzable aqueous extract of ragweed pollen (DWSR) was separated by zone electrophoresis on Sephadex G-25 into fractions differing in their antigenic composition. The skin activities of the electrophoretic fractions were shown to depend on both the allergio serum and the volunteer used for the Prausnitz-Kustner (P-K) tests. Maximal wheal and flare reactions were obtained in P-K tests on interaction of fractions of allergic serum with fractions of DWSR of opposite electrical charge. Skin-sensitizing antibodies were found to differ in their affinity for normal skin. The cross-neutralization of P-K reactions with three allergic sera revealed: (1) the presence of at least five distinct allergenic constituents, and (2) a different spectrum of skin-sensitizing antibodies for the three allergic sera directed against one or more of the allergenic constituents.
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